Mod Spec® Service の概要
Mod Spec® は、質量分析計を使って60種類以上の異なるヒストン修飾の相対量を一度に解析する手法です. Mod Spec® は、エピジェネティックな変化が疾患や薬物処理に応答して生じているかどうかを判断するための出発点になります。多くの修飾変化を一度に見出すことで、予想された変化の確認だけでなく、予期しない重要な変化などを発見できることも期待できます。
日本語チラシはこちら。
ヒストン修飾変化の解析例:
- エピジェネティックな阻害剤への反応
- 正常細胞と罹患細胞の比較
- Knock-out 細胞 や動物細胞
- 薬剤処理した移植サンプル
- ヒト生検サンプル
How Does Mod Spec® Work?
1. Histone Extraction: From 2-5 million cells or 25-100 mg tissue.
2. Propionylation: Mass spectrometry of proteins requires digestion of the proteins into small peptides before injection into the mass spectrometer. Propionylation, using propionic anhydride, blocks cleavage at lysines by trypsin, while allowing digestion at arginines to occur. The resulting peptides are of the appropriate size for mass spec analysis.
3. Trypsin Digestion: Histones are digested with trypsin.
4. Mass Spec: Samples are analyzed on a Triple-Stage Quadrupole Mass Spectrometer.
- Q1: Peptides of the appropriate molecular weight are selected.
- Q2: Peptides are further fragmented in the collision chamber.
- Q3: Peptides fragments of interest are selected and analyzed by mass spectrometry.
5. Analysis: Fragments are analyzed and graphed to show changes between samples.
Histone Modifications Detected by Mod Spec®
^ Multiple H2A isoforms may contribute to the signal for modifications on H2A.
* H3R2me2 and H3K4me2/3 are mutually exclusive modifications. H3R2 methylation prevents H3K4 methylation. Therefore, H3K4 modifications are reported only on the H3R2 unmodified peptide. For more information, see Nature. 2007 Oct 18; 449(7164):933-7.
♦ H3.2 may contribute to signals for modifications labeled H3.1.
What our customers are saying about us:
"While working on the molecular mechanism of an epigenetic drug, we outsourced Mod Spec® to Active Motif in order to get a broader overview of the drug-induced changes of histone modifications. Overall, we were very pleased with the quality of the service, the kept timeline and last but not least, the fair price. We found surprising things that were not on our radar before."
Matthias Lauth, PhD
Phillips University
Marburg, Germany
View complete list of testimonials >
Mod Spec® 受託解析サービスのデータ
Figure 1: Mod Spec® data from control HeLa cells and HeLa cells treated for 7 days with 0.5uM GSK-126.
Figure 2: Mod Spec® data from control HEK293 cells and HEK293 cells treated for 6 hours with 5 mM sodium butyrate (NaB).
Mod Spec® Service 使用文献
Search our database of customer publications that have used our Mod Spec® services.
Mod Spec® 受託解析サービスの資料
Mod Spec® Service Sample Submissionポータル
オンラインSample Submissionポータルは、解析したいサンプルの情報をアクティブ・モティフの担当者と共有し、依頼者には解析の進捗をご覧いただけるサイトです。 解析を依頼するために必要な連絡先、サンプルの情報、また解析の内容を記載していただきます。記載の方法や、サンプル提出方法について、ご不明な点がありましたら「[email protected]」までご連絡ください。
こちらもご参照ください:
Name | Cat No. | Price | |
---|---|---|---|
Mod Spec® | 25085 | Get Quote |