YOUR CART
Reporter gene assays are an important tool used for studying signal transduction pathways because of their simplicity and versatility. However, standard reporter assays are limited by the fact that basal activity of the cloned promoter ressults in accumulation of luciferase mRNA and protein. The slow clearance rate of these reporter molecules substantially delays and dilutes the measurable response to stimulation or repression. As a result, with standard reporter assays, transient or relatively minor effects are hidden and kinetic assays are inaccurate.
Active Motif's patented RapidReporter®* method overcomes these limitations by utilizing vectors that include both protein and mRNA destabilizing elements. This causes the cell to rapidly degrade both the luciferase protein and mRNA, which lowers background and increases both the magnitude of the response that can be measured after stimulation or repression, and the speed with which the assay can report changes in transcription. In addition, RapidReporter uses a modified Gaussia luciferase, the most sensitive luciferase known. The combination of Gaussia expression and double destabilization means that RapidReporter enables researchers to detect smaller changes in activity than is possible using non-destabilized reporter gene assays, or those that destabilize the protein only. Click the link on the right for more information.
