Recombinant Histones

Active Motif offers Recombinant Histones to study site- and degree-specific methylation as well as acetylation modifications.

Histones with specific methylation states are an essential tool for investigating which methylation patterns are key to complex functional questions about chromatin-associated proteins, nucleosome remodeling, transcriptional regulation, replication and DNA repair. Click on our link to get a complete list of our Recombinant Methylated Histones.

In addition to methylation specific recombinant histones, Active Motif also offers histones with site-specific acetylation modifications. Histone acetylation is a post-translational modification that affects the nucleosome structure and therefore the ability of transcription factors to access the DNA and regulate gene expression. Click on our link to get a complete list of our Recombinant Acetylated Histones.

Histone phosphorylation at serine and threonine residues can influence transcription, chromosome condensation, DNA repair and apoptosis. Active Motif offers Recombinant Histones to study site-specific histone phosphorylation. Click on our link to get a complete list of our Recombinant Phosphorylated Histones.

Active Motif has now added Recombinant Histone H3 biotinylated protein to it's portfolio of recombinant histones. The recombinant H3 protein is linked to biotin at the N-terminus via a carbon linker. The addition of the biotin enables capture of protein binding interactions utilizing the recombinant histone as a substrate. The biotinylated histone H3 protein is also an ideal substrate for homogenous FRET assays. Simply incubate the recombinant histone with the enzyme of interest and detect using streptavidin-coated donor beads and antibody-conjugated acceptor beads. Click on our link to learn more about the Recombinant Histone H3 biotinylated.