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Histones with specific methylation states are an essential tool for investigating which methylation patterns are key to complex functional questions about chromatin-associated proteins, nucleosome remodeling, transcriptional regulation, replication and DNA repair.
Recombinant methylated histones are created via a chemical alkylation reaction that introduces an analog of methyl lysine. This specific chemical treatment enables the site and degree of methylation to be precisely controlled. Each methylation reaction is over 99% complete, as verified by high-resolution ESI-TOF mass spectrometry. All recombinant histones are confirmed by SDS-PAGE and dot blot or immunoblot. The methylation state of these recombinant histones closely resembles natural methylation, so these histones are perfect for any functional assay.
Figure 1: Western blot analysis of mono-, di- and tri-methylated Recombinant Histone H3.
One µg Recombinant Histone H3 (Lanes 1, 5. & 9), 1 µg Recombinant Histone H3 monomethyl Lys9 (Lanes 2, 6 & 10), 1 µg Recombinant Histone H3 dimethyl Lys9 (Lanes 3, 7 & 11) and 1 µg Recombinant Histone H3 trimethyl Lys9 (Lanes 4, 8 & 12) probed with anti-Histone H3 monomethyl Lys9 (Cat. No. 39249) (Lanes 1 - 4), anti-Histone H3 dimethyl Lys9 (Cat. No. 39239) (Lanes 5 - 8) and anti-Histone H3 trimethyl Lys9 (Cat. No. 39161) (Lanes 9 - 12).
Contents & Storage
50 µg supplied as lyophilized powder. Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. Products are guaranteed stable for 6 months after receipt when stored properly.
