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Histone phosphorylation occurs on serine and threonine residues and influences transcription, chromosome condensation, DNA repair and apoptosis. The recombinant phosphorylated histones are made using our patent pending technology, which enables phosphorylation of the histone tail without altering the native peptide bond
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| Recombinant Histone H2A | 50 µg | 31251 | $345 | Buy Now |
| Recombinant Histone H2B | 50 µg | 31252 | $345 | Buy Now |
| Recombinant Histone H3 (C110A) | 100 µg | 31207 | $345 | Buy Now |
| Recombinant Histone H3 biotinylated | 25 µg | 31271 | $345 | Buy Now |
| Recombinant Histone H3 phospho Thr3 (H3T3ph) | 25 µg | 31274 | $345 | Buy Now |
| Recombinant Histone H3 phospho Ser10 (H3S10ph) | 25 µg | 31272 | $345 | Buy Now |
| Recombinant Histone H4 | 50 µg | 31223 | $345 | Buy Now |
Recombinant phosphorylated histones are created using a patent pending technology that introduces a site specific phosphorylation on the histone tail without altering the native peptide bonds. Each recombinant phosphorylated protein is over 99% pure, as verified by high-resolution ESI-TOF mass spectrometry. All recombinant histones are confirmed by SDS-PAGE and dot blot. These recombinant histones are equivalent to native phosphorylated histones, so these proteins are perfect for functional assays or for use as positive controls for phosphorylation detection.
Figure 1: SDS-PAGE gel analysis of Recombinant Histone H3 phospho Thr3.
An SDS-PAGE gel was run with 0.5 µg Recombinant Histone H3 phospho Thr3 (Lane 2), 1 µg Recombinant Histone H3 phospho Thr3 (Lane 3) and a protein marker (Lane 1).
Contents & Storage
25 µg supplied as lyophilized powder. Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. Products are guaranteed stable for 6 months after receipt when stored properly.
