Active Motif,
Tools to analyze nuclear function,
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Hydroxymethyl Collector™

biotin-based enrichment of 5-hydroxymethylcytosine

The Hydroxymethyl Collector™ Kits are designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation* for analysis. They utilize a β-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin magnetic beads.

* Song, C.X., et al., Nature Biotechnology. 29, 68-72 (2011).
Patent US 8,741,567 B2.

Active Motif's Hydroxymethyl Collector and Hydroxymethyl Collector-Seq Kits are currently unavailable.

Please check out our hMeDIP Kit (Catalog No. 55010) as an alternative for the enrichment of DNA fragments containing 5-hydroxymethylcytosine using antibody-based enrichment.

 
Name Format Cat No. 価格 (税抜)  
UDP-Azide-Glucose 1 vial 55020 ¥42,000 Buy Now