LightSwitch™ Reporter System

LightSwitch™ Promoter Reporter GoClone™ Collection

The Promoter Reporter Collection includes over 18,000 promoters cloned from the human genome and available as transfection-ready GoClone constructs. So, you don't need to spend time and effort cloning the promoters needed for your transcriptional regulation studies. Simply use our Search Tool to find vectors with your promoters of interest, then transfect and assay their activity immediately.

LightSwitch™ Promoter
Reporter Vector.

LightSwitch™ 3´UTR Reporter GoClone™ Collection &
miRNA Mimics and Inhibitors

The 3´UTR Reporter Collection includes over 12,000 3´UTRs cloned from the human genome into ready-to-transfect LightSwitch reporter vectors. Combined with our large collection of miRNA Mimics and Inhibitors, you have everything needed to study miRNA-3´UTR interactions, validate miRNA targets, measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis.

LightSwitch™ 3´UTR
Reporter Vector.

LightSwitch™ lncRNA Promoter Reporter Collection

While long noncoding RNAs (lncRNAs) have been shown to play important roles in the regulation of both transcription and chromatin organization, the mechanisms by which their own expression is regulated seem to differ from those of protein-coding genes. The LightSwitch lncRNA Promoter Reporter Collection was designed to make it fast & easy to study the regulation of lncRNA promoters.

LightSwitch™ Promoter
Reporter Vector.

LightSwitch™ Validated
Pathway Collections

The Validated Pathway Collections are subsets of our LightSwitch Promoter constructs that were selected based on sequence motif analysis and published information, followed by in-house testing using our LightSwitch Luciferase Assay. All members have been experimentally validated to show significant activation or repression in response to pathway-specific induction conditions, so they are ideal when studying pathways such as inflammation, hypoxia, DNA damage, heat shock, etc.

LightSwitch™ Validated
Pathway Collections.

Synthetic Response Elements &
Synthetic miRNA Targets

Because they have been engineered for increased sensitivity, the use of synthetic regulatory elements can improve some regulation experiments greatly. The stronger responses provided by our optimized LightSwitch Synthetic Response Elements and LightSwitch Synthetic miRNA Target Reporters can yield more meaningful data than that from native, endogenous promoter and 3´UTR sequences.

Synthetic Enhancers &
miRNA Targets.

LightSwitch™ Custom Services

In addition to the many different products that make up the LightSwitch Luciferase Assay System, we offer a wide variety of custom services, including:

• Custom Cloning
• Custom Mutagenesis
• Pathway Screening Services
• Sequence Variant Assay Services
• miRNA Target Validation Services

Our Custom Services Lab Can
Accelerate Your Research.

LightSwitch™ Pathway Reporter
Stable Cell Lines

Each LightSwitch Pathway Reporter Stable Cell Line contains a stably integrated, human regulatory element driving the expression of our optimized RenSP luciferase gene. This makes it far easier to measure the activity of specific pathways in response to various treatments and growth conditions. Each cell line has been validated using a variety of different plate-based formats, making them particularly useful for high-throughput screening experiments.

LightSwitch™ Pathway Reporter
Stable Cell Lines.

Transfection Reagents

After selecting a LightSwitch reporter construct, you must get it into your cell line. For plasmid transfections and co-transfections, we offer FuGENE^® HD Transfection Reagent because of its consistent, high-quality performance across a wide variety of cell lines. For co-transfecting plasmids and miRNAs, we offer DharmaFECT^® Duo Transfection Reagent, which provides more efficient co-transfection of those two distinct types of molecules. We also offer the LightSwitch Transfection Optimization Kit to quickly determine the conditions needed for high-efficiency transfections of your specific cell lines.

Mammalian cell transfection.

LightSwitch Assay Reagents

After transfection, the amount of luciferase produced is measured using our LightSwitch Luciferase Assay Kit. Because RenSP catalyzes oxidation of its substrate in a reaction that produces light, gene expression can be quantified using a luminometer. The novel, proprietary substrate and optimized lysis buffer included in the LightSwitch Luciferase Assay Kit were formulated to provide high sensitivity over a broad dynamic range when used with our engineered RenSP. For those experiments that utilize co-transfection for normalization, we offer our LightSwitch Dual Assay Kit.

Light produced by luciferase.

LightSwitch Controls & Vectors

To help ensure you get the best results possible, we have developed panels of LightSwitch Promoter Control Vectors and LightSwitch 3´UTR Control Vectors. These positive & negative control constructs make it easier to interpret your data and to troubleshoot your work should things not go as expected. In addition, we offer Empty LightSwitch Reporter Vectors, which are useful as controls and for cloning your own regulatory elements.

Positive & negative promoter controls.