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ATTO 647N (STED/GSD) Goat anti-Rabbit IgG

商品コード: 15048 Format: 250 µl ¥49,000 Buy Now
商品コード: 15068 Format: 35 µl ¥26,000 Buy Now
Application:Immunofluorescence
Absorption:644 nm
Emission:669 nm
Molar Extinction:150,000 M-1 cm-1
Quantum Yield:~65%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Rabbit
Purification:Affinity Purified

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for ATTO 647N (STED/GSD) Goat anti-Rabbit IgGContents

Contents: 250 µl (Catalog No. 15048) or 35 µl (Catalog No. 15068) of affinity purified goat anti-rabbit IgG (H+L) conjugated to ATTO 647N (STED/GSD) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule rabbit IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, mouse, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

ATTO 647N (STED/GSD) belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photo stability, excellent ozone resistance, good solubility, and very little triplet formation.

for ATTO 647N (STED/GSD) Goat anti-Rabbit IgGApplication Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

ATTO 647N (STED/GSD) Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leica's STimulated Emission Depletion (STED) microscopes. It is also recommended by Leica for use in Ground State Depletion with Individual Molecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of ATTO 647N (STED/GSD) Dye.

Absorption and emission spectra of ATTO 647N (STED/GSD) Dye.

Immunofluorescence staining using the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody

Immunofluorescence staining using the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody.
HeLa cells stained with Histone H3 trimethyl Lys27 rabbit polyclonal antibody (Cat. No. 39155) at 1:500 dilution and ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody at 1:1,000. Green: Nucleolin mouse monoclonal antibody (Cat. No. 39541).

Active Motif's primary antibodies and fluorescent secondary antibodies in STED microscopy.

Active Motif's primary antibodies and fluorescent secondary antibodies in STED microscopy.
HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527) and Chromeo 494 Goat anti-Mouse IgG (Cat. No. 15032) secondary antibody. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Cat. No. 39159) and the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody. This STED (STimulated Emission Depletion) image is courtesy of Leica Microsystems, Germany.

for ATTO 647N (STED/GSD) Goat anti-Rabbit IgGStorage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATTO 647N (STED/GSD) Goat anti-Rabbit IgGGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

ATTO 647N (STED/GSD) Goat anti-Rabbit IgG
 

Data Thumbnails

Absorption and emission spectra of ATTO 647N (STED/GSD) Dye.

Absorption and emission spectra of ATTO 647N (STED/GSD) Dye.
(Click image to enlarge and see details.)

Immunofluorescence staining using the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody

Immunofluorescence staining using the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody.
(Click image to enlarge and see details.)

Active Motif's primary antibodies and fluorescent secondary antibodies in STED microscopy.

Active Motif's primary antibodies and fluorescent secondary antibodies in STED microscopy.
(Click image to enlarge and see details.)