Recombinant NFκB p65 protein

for Recombinant NFkB p65 proteinContents

5 µg of Recombinant NFκB p65 protein 1X Protein Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl₂ and 10% glycerol) and 1 M DTT. Protein is supplied at 100 ng/µl in Protein Dilution Buffer.

for Recombinant NFkB p65 proteinApplication Notes

Recombinant NFκB p65 is suitable for Western blotting and TransAM^® assays. 100 ng is sufficient for most protein-protein interaction studies. The standard curve for TransAM NFκB p65 was generated using the range of 40-0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

for Recombinant NFkB p65 proteinProtein Details

Recombinant NFκB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration.

for Recombinant NFkB p65 proteinStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant NFkB p65 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.