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Recombinant NFκB p65 protein

Aliases: RELA, p65, NFKB3
商品コード: 31102 Format: 5 µg ¥73,000 Buy Now
商品コード: 31302 Format: 15 µg ¥157,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant NFkB p65 proteinContents

5 µg of Recombinant NFκB p65 protein, 1X Protein Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl2 and 10% glycerol) and 1 M DTT. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant NFkB p65 proteinBackground

NFκB p65 is a subunit of the NFκB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. NFκB p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.

for Recombinant NFkB p65 proteinApplication Notes

Recombinant NFκB p65 is suitable for Western blotting and TransAM® assays. 100 ng is sufficient for most protein-protein interaction studies. The standard curve for TransAM NFκB p65 was generated using the range of 40-0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

for Recombinant NFkB p65 proteinProtein Details

Recombinant NFκB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration.

 
TransAM<sup>®</sup> standard curve using Recombinant NFκB p65 protein.

TransAM® standard curve generated using Recombinant NFκB p65 protein.
The standard curve for TransAM® was generated using a range of 40 - 0.625 ng of protein and run on the TransAM® NFκB p65 ELISA Kit.

for Recombinant NFkB p65 proteinStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant NFkB p65 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant NFκB p65 protein
 

Data Thumbnails

TransAM<sup>®</sup> standard curve using Recombinant NFκB p65 protein.

TransAM® standard curve using Recombinant NFκB p65 protein.
(Click image to enlarge and see details.)