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Recombinant p53 protein

Aliases: TP53, BCC7, LFS1, TRP53
商品コード: 31103 Format: 5 µg ¥86,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant p53 proteinContents

5 µg of Recombinant p53 protein and 1X Protein Dilution Buffer (20 mM Tris-HCl pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA and 1 mM DTT. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant p53 proteinBackground

p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.

for Recombinant p53 proteinApplication Notes

Recombinant p53 is suitable for TransAM®, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40 – 0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

for Recombinant p53 proteinProtein Details

Full-length recombinant p53 protein was expressed in a baculovirus system with a His-Tag and purified by an affinity column in combination with FPLC chromatography.

Active Motif also offers Recombinant p53 protein (Catalog No. 31465) that was expressed in E. coli.

 
TransAM<sup>®</sup> standard curve using Recombinant p53 protein.

TransAM® standard curve generated using Recombinant p53 protein.
The standard curve for TransAM® was generated using a range of 40 – 0.625 ng of protein and run on the TransAM® p53 ELISA Kit.

for Recombinant p53 proteinStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant p53 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant p53 protein
 

Data Thumbnails

TransAM<sup>®</sup> standard curve using Recombinant p53 protein.

TransAM® standard curve using Recombinant p53 protein.
(Click image to enlarge and see details.)