Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant PPARγ protein

Aliases: GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma
商品コード: 31127 Format: 10 µg ¥72,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant PPARg proteinContents

10 µg of Recombinant PPARγ protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PPARg proteinBackground

Peroxisome Proliferator-Activated Receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: α, δ and ϒ. PPARγ stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs bind to peroxisome proliferator response elements (PPREs) as heterodimers with the retinoid X receptor (RXR).

for Recombinant PPARg proteinApplication Notes

Recombinant PPARγ is suitable for DNA and protein-protein interaction assays. 20 ng are sufficient for gelshift assays and 100 ng are sufficient for protein-protein interaction studies. The molecular weight of the protein is ~60 kDa. This protein is not recommended for TransAM®. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

for Recombinant PPARg proteinProtein Details

Recombinant PPARγ is isolated from an E. coli strain that carries the coding sequence of the human PPARγ under the control of a T7 promoter (accession number NM 138712). The purified recombinant protein has an N-terminal His-Tag and is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.

 
PPARγ Coomassie gel

PPARγ protein gel.
PPARγ protein run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant PPARg proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PPARγ protein
 

Data Thumbnails

PPARγ Coomassie gel

PPARγ Coomassie stained gel.
(Click image to enlarge and see details).