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for Recombinant Sp1 proteinContents
5 µg of Recombinant Sp1 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.4 µg/µl in Dilution Buffer AM1.
for Recombinant Sp1 proteinApplication Notes
Recombinant Sp1 is suitable for in vitro transcription, DNase I protection, gelshift and TransAM™ assays. 30-100 ng is sufficient for reconstituted transcription assays and 5-25 ng in a 20 µl volume are sufficient for gelshift assays. The molecular weight of the protein is ~100 kDa. The standard curve for TransAM® Sp1 was generated using the range of 320-40 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant Sp1 proteinProtein Details
The wild-type Sp1 protein (785 amino acids, accession number NM 138473) was expressed in a baculovirus system with an amino terminal polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
TransAM® standard curve generated using Recombinant Sp1 protein.
The standard curve for TransAM® was generated using a range of 320-40 ng of protein and run on the TransAM® Sp1 ELISA Kit.
for Recombinant Sp1 proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
