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Recombinant ABL1 protein

Aliases: ABL, c-Abl, JTK7, p150, v-Abl, bcr/abl, c-ABL1
商品コード: 31160 Format: 10 µg ¥71,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant ABL1 proteinContents

10 µg of Recombinant ABL1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant ABL1 proteinApplication Notes

Recombinant ABL1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.14 kDa. The activity of the protein is ~ 33 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY, 5 µg/50 µl, Recombinant ABL1: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant ABL1 proteinProtein Details

Recombinant human ABL1 protein was produced using baculovirus infected Sf9 cells. The protein was against amino acids P118-S535, accession number M14752 and N-terminally fused to GST-Factor Xa cleavage site. Purified by GST-agarose affinity purification.

 
Recombinant ABL1 protein, active, analyzed by SDS-PAGE gel.

Recombinant ABL1 protein tested by SDS-PAGE gel.
0.5 µg of ABL1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-ABL1 or anti-GST antibody.

for Recombinant ABL1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant ABL1 protein
 

Data Thumbnails

Recombinant ABL1 protein, active, analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant ABL1 protein, active.
(Click image to enlarge and see details.)