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Recombinant CSK protein

Aliases: C-Src Tyrosine Kinase
商品コード: 31164 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant CSK proteinContents

10 µg of Recombinant CSK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant CSK proteinApplication Notes

Recombinant CSK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~80.215 kDa. The activity of the protein is ~ 83 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant CSK: 40 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant CSK proteinProtein Details

Recombinant human CSK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-L450 accession number NM_004383 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant CSK protein, active analyzed by SDS-PAGE gel.

Recombinant CSK protein tested by SDS-PAGE gel.
0.5 µg of CSK protein was analyzed using SDS/PAGE followed by Western blot with anti-GST antibody.

for Recombinant CSK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant CSK protein
 

Data Thumbnails

Recombinant CSK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant CSK protein, active.
(Click image to enlarge and see details.)