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Recombinant EGFR protein

Aliases: ERBB, HER1, mENA, ERBB1, PIG61, NISBD2
商品コード: 31165 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant EGFR proteinContents

10 µg of Recombinant EGFR protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant EGFR proteinApplication Notes

Recombinant EGFR is suitable for kinase assays and Western blot. The molecular weight of the protein is ~91.002 kDa. The activity of the protein is ~ 93 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 10 µg/50 µl, Recombinant EGFR: 40 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant EGFR proteinProtein Details

Recombinant human EGFR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids H672-A1210, accession number NM_005228 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant EGFR protein, active analyzed by SDS-PAGE gel.

Recombinant EGFR protein tested by SDS-PAGE gel.
0.5 µg of EGFR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-EGFR or anti-GST antibody.

for Recombinant EGFR proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant EGFR protein
 

Data Thumbnails

Recombinant EGFR protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant EGFR protein, active.
(Click image to enlarge and see details.)