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Recombinant HCK protein

Aliases: JTK9, p59Hck, p61Hck, HCK Proto-Oncogene, Src Family Tyrosine Kinase
商品コード: 31174 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HCK proteinContents

10 µg of Recombinant HCK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HCK proteinApplication Notes

Recombinant HCK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~86.707 kDa. The activity of the protein is ~ 34 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant HCK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant HCK proteinProtein Details

Recombinant human HCK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-P505, accession number NM_002110 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant HCK protein, active analyzed by SDS-PAGE gel.

Recombinant HCK protein tested by SDS-PAGE gel.
0.5 µg of HCK protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody.

for Recombinant HCK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HCK protein
 

Data Thumbnails

Recombinant HCK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant HCK protein, active.
(Click image to enlarge and see details.)