Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant IKKβ protein

Aliases: IKK2, IKBKB, IMD15, NFKBIKB, IKK-beta
商品コード: 31176 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant IKKb proteinContents

10 µg of Recombinant IKKβ protein in 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant IKKb proteinBackground

IKKβ (IκB Kinase beta subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB.

While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα. Once activated, IKKβ autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.

for Recombinant IKKb proteinApplication Notes

Recombinant IKKβ is suitable for kinase assays and Western blot. The molecular weight of the protein is 120.201 kDa. The activity of the protein is 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, 50 μg/ml PEG20.000, ATP (variable), Substrate: IκBα derived peptide (R11-DDRHDSGLDSMKD) at 40 μg/ml, Recombinant IKK&beta at 2 μg/ml. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant IKKb proteinProtein Details

Recombinant human IKKβ protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-S756, accession number NM_001556 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by Immobilized Metal Affinity Chromatography.

 
Recombinant IKKβ protein, active analyzed by Coomassie.

Recombinant IKKβ protein Coomassie Stain
Coomassie stained gel of 2 µg of IKKβ protein.

IKKβ Kinase Activity Assay

IKKβ activity assay.
Recombinant IKKβ activity measured using a kinase assay.

for Recombinant IKKb proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant IKKb proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant IKKβ protein
 

Data Thumbnails

Recombinant IKKβ protein, active analyzed by Coomassie.

Coomassie stain gel of Recombinant IKKβ protein, active.
(Click image to enlarge and see details.)

IKKβ Kinase Activity Assay

IKKβ Kinase Activity Assay.
(Click image to enlarge and see details.)