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Recombinant IRAK-4 protein

Aliases: IPD1, REN64, IRAK-4, NY-REN-64
商品コード: 31179 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant IRAK-4 proteinContents

10 µg of Recombinant IRAK-4 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant IRAK-4 proteinApplication Notes

Recombinant IRAK-4 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~69.898 kDa. The activity of the protein is ~ 574 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Histone H2B 5 µg/50 µl, Recombinant IRAK-4 : 20 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant IRAK-4 proteinProtein Details

Recombinant human IRAK-4 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids A104-S460, accession number AF445802 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant IRAK-4 protein, active analyzed by SDS-PAGE gel.

Recombinant IRAK-4 protein tested by SDS-PAGE gel.
0.5 µg of IRAK-4 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-IRAK-4 or anti-GST antibody.

for Recombinant IRAK-4 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant IRAK-4 protein
 

Data Thumbnails

Recombinant IRAK-4 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant IRAK-4 protein, active.
(Click image to enlarge and see details.)