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Recombinant Lyn protein

Aliases: LYN Proto-Oncogene, Src Family Tyrosine Kinase, JTK8, p53Lyn, p56Lyn
商品コード: 31185 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant Lyn proteinContents

10 µg of Recombinant Lyn protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Lyn proteinApplication Notes

Recombinant Lyn is suitable for kinase assays and Western blot. The molecular weight of the protein is ~88.47 kDa. The activity of the protein is ~ 94 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 2 µg / 50 µl, Recombinant Lyn : 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant Lyn proteinProtein Details

Recombinant human Lyn protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-P512, accession number NM_002350 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant Lyn protein, active analyzed by SDS-PAGE gel.

Recombinant Lyn protein tested by SDS-PAGE gel.
0.5 µg of Lyn protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody.

for Recombinant Lyn proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Lyn protein
 

Data Thumbnails

Recombinant Lyn protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant Lyn protein, active.
(Click image to enlarge and see details.)