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Recombinant PAK2 protein

Aliases: P21 (RAC1) Activated Kinase 2, PAK65, PAKgamma, PAKgamma
商品コード: 31188 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PAK2 proteinContents

10 µg of Recombinant PAK2 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PAK2 proteinBackground

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

for Recombinant PAK2 proteinApplication Notes

Recombinant PAK2 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~87.954 kDa. The activity of the protein is ~ 228 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 5 µg / 50 µl, Recombinant PAK2 : 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant PAK2 proteinProtein Details

Recombinant human PAK2 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids D3-R534, accession number NM_002577 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant PAK2 protein, active analyzed by SDS-PAGE gel.

Recombinant PAK2 protein tested by SDS-PAGE gel.
0.5 µg of PAK2 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PAK2 or anti-GST antibody.

for Recombinant PAK2 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PAK2 protein
 

Data Thumbnails

Recombinant PAK2 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant PAK2 protein, active.
(Click image to enlarge and see details.)