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Recombinant PAK4 protein

Aliases: P21 (RAC1) Activated Kinase 4
商品コード: 31189 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PAK4 proteinContents

10 µg of Recombinant PAK4 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PAK4 proteinBackground

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

for Recombinant PAK4 proteinApplication Notes

Recombinant PAK4 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~93.968 kDa. The activity of the protein is ~ 8 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 2.5 µg / 50 µl, Recombinant PAK4: 100 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant PAK4 proteinProtein Details

Recombinant human PAK4 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-R591, accession number NM_005884 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant PAK4 protein, active analyzed by SDS-PAGE gel.

Recombinant PAK4 protein tested by SDS-PAGE gel.
0.5 µg of PAK4 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PAK4 or anti-GST antibody.

for Recombinant PAK4 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PAK4 protein
 

Data Thumbnails

Recombinant PAK4 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant PAK4 protein, active.
(Click image to enlarge and see details.)