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Recombinant PDGFRα protein

Aliases: Platelet Derived Growth Factor Receptor Alpha, CD140A, PDGFR-2
商品コード: 31190 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PDGFRa proteinContents

10 µg of Recombinant PDGFRα protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PDGFRa proteinBackground

PDGFRα (Platelet Derived Growth Factor Receptor alpha) is a tyrosine protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. PDGFRα may promote or inhibit cell proliferation and migration, and plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. It is required for normal embryonic development of several tissue and organ types and plays a role in cell migration and chemotaxis in wound healing and platelet activation. Binding of its ligands - homodimers or heterodimers of PDGFA and PDGFB leads to the activation of several signaling cascades.

for Recombinant PDGFRa proteinApplication Notes

Recombinant PDGFRα is suitable for kinase assays and Western blot. The molecular weight of the protein is ~87.851 kDa. The activity of the protein is ~ 10 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY 2.5 µg / 50 µl, Recombinant PDGFRα: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant PDGFRa proteinProtein Details

Recombinant human PDGFRα protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids Q551-L1089, accession number NM_006206 and N-terminally fused to GST-Factor Xa cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant PDGFRα protein, active analyzed by SDS-PAGE gel.

Recombinant PDGFRα protein tested by SDS-PAGE gel.
0.5 µg of PDGFRα protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PDGFRα or anti-GST antibody.

for Recombinant PDGFRa proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PDGFRα protein
 

Data Thumbnails

Recombinant PDGFRα protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant PDGFRα protein, active.
(Click image to enlarge and see details.)