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Recombinant SNK protein

Aliases: PLK2, hSNK, hPlk2
商品コード: 31194 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant SNK proteinContents

10 µg of Recombinant SNK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SNK proteinApplication Notes

Recombinant SNK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~107.435 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF, 10 µg / 50 µl, Recombinant SNK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant SNK proteinProtein Details

Recombinant human SNK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-N685, accession number NM_006622 and N-terminally fused to GST-HIS6-Thrombin site. Purified by GSH-agarose affinity purification.

 
Recombinant SNK protein, active analyzed by SDS-PAGE gel.

Recombinant SNK protein tested by SDS-PAGE gel.
0.5 µg of SNK protein was analyzed using SDS/PAGE followed by Western blot with either an anti-GSK or anti-SNK antibody.

for Recombinant SNK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant SNK protein
 

Data Thumbnails

Recombinant SNK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant SNK protein, active.
(Click image to enlarge and see details.)