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Recombinant SYK protein

Aliases: p72-Syk
商品コード: 31196 Format: 10 µg ¥72,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant SYK proteinContents

10 µg of Recombinant SYK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SYK proteinApplication Notes

Recombinant SYK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~104.408 kDa. The activity of the protein is ~ 12 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 2 µg / 50 µl, Recombinant SYK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant SYK proteinProtein Details

Recombinant human SYK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-N635, accession number NM_003177 and N-terminally fused to GST-HIS6-Thrombin site. Purified by GSH-agarose affinity purification.

for Recombinant SYK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant SYK protein