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for Recombinant p300, catalytic domainContents
5 µg of Recombinant p300, catalytic domain. 20 mM Tris-HCl pH 7.3, 20% glycerol, 0.2 mM EDTA, 300 mM KCl, 1 mM DTT containing 0.5 mM PMSF.
for Recombinant p300, catalytic domainApplication Notes
This protein has been tested for activity in the fluorescent HAT Assay Kit, Catalog No. 56100. 50 ng of p300 were assayed per well using 50 µM H3 and H4 peptides, 50 µM Acetyl-CoA, and 15 µM Anacardic Acid. Assays were incubated for 30 minutes at room temperature and read on a fluorescent plate reader (ex 360-390 nm, em 450-470 nm). The specific activity was >500 pmol/min/µg.
for Recombinant p300, catalytic domainProtein Details
Recombinant p300 catalytic domain is expressed in a baculovirus system and purified by affinity chromatography. Protein is supplied at 0.1 mg/ml. The p300 corresponds to amino acids 965-1810 from the GenBank Accession Number U01877.
Recombinant p300 protein activity assay.
p300 activity using the fluorescent HAT assay kit (Catalog No. 56100). p300 was tested on both histone H3 (residues 5-23 of human H3) and H4 peptide (residues 1-19 of human H4) substrates. p300 activity was inhibited by anacardic acid.
for Recombinant p300, catalytic domainStorage
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant p300, catalytic domainGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
