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for Recombinant Histone H4Contents
50 µg supplied as lyophilized powder.
for Recombinant Histone H4Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
for Recombinant Histone H4Application Notes
Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly. Recombinant Histone H4 is useful as a wild-type control for assays including applications that use recombinant methylated histones.
for Recombinant Histone H4Protein Details
Recombinant Xenopus laevis Histone H4 produced in E. coli and purified using FPLC. Protein concentration was determined by spectrophotometry. ≥98% purity by SDS-PAGE.
Recombinant Histone H4 analyzed by SDS-PAGE gel.
SDS-PAGE analysis of 1.5 µg Recombinant Histone H4 (lane 2), Recombinant Histone H4 monomethyl Lys20 (lane 3), Recombinant Histone H4 dimethyl Lys20 (lane 4), and Recombinant Histone H4 trimethyl Lys20 (lane 5). Molecular weight marker is in lane 1.
for Recombinant Histone H4Storage
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant Histone H4Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
