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Recombinant Ubiquitin G76A protein

商品コード: 31232 Format: 10 µg ¥72,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant Ubiquitin G76A proteinContents

10 µg of recombinant protein supplied at a concentration of 0.5 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant Ubiquitin G76A proteinBackground

Ubiquitin is a small, highly conserved regulatory protein that exists in all eukaryotic cells. Ubiquitin is covalently attached to a range of target proteins in order to mark these proteins for proteasomal degradation. Ubiquitination involves three classes of enzymes: E1s, ubiquitin-activating enzymes, E2s, ubiquitin-conjugating enzymes and E3s, ubiquitin-protein ligases.

for Recombinant Ubiquitin G76A proteinApplication Notes

Recombinant Ubiquitin G76A protein is suitable for use in ubiquitination assays as a negative control. A recommended starting point for the ubiquitination assay is 200-500 ng recombinant protein per reaction.

for Recombinant Ubiquitin G76A proteinProtein Details

Recombinant Ubiquitin G76A protein was expressed in E. coli as amino acids 1-76 of human protein (accession number AAA72608) with an N-terminal His-Tag. Recombinant ubiquitin G76A protein contains a substitution of a glycine residue to alanine at amino acid 76. The molecular weight of the protein is ~10.6 kDa.

for Recombinant Ubiquitin G76A proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant Ubiquitin G76A protein