Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant Histone H3K27ac

商品コード: 31290 Format: 25 µg ¥74,000 Buy Now
Expressed In: Synthetic Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant Histone H3K27acContents

25 µg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

for Recombinant Histone H3K27acBackground

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3K27acApplication Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3K27acProtein Details

Recombinant Histone H3K27ac proteins are synthetic modified histones containing acetylation at K27. The Recombinant Histone H3K27ac proteins correspond to the native histone sequence and do not contain any amino acid substitutions or residue analogs. Protein concentration was determined using Bradford assay. The recombinant histone is >95% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,313 Daltons. To see a representative mass spectrum, please visit our website at www.activemotif.com and view the product details page.

 
SDS-PAGE gel analysis of Recombinant Histone H3K27ac protein.

SDS-PAGE gel analysis of Recombinant Histone H3K27ac protein.

for Recombinant Histone H3K27acStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in stora

for Recombinant Histone H3K27acGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Histone H3K27ac
 

Data Thumbnails

SDS-PAGE gel analysis of Recombinant Histone H3K27ac protein.