Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant SIRT1 protein

Aliases: SIR2; hSIR2; SIR2L1
商品コード: 31340 Format: 10 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant SIRT1 proteinContents

Recombinant SIRT1 protein supplied in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SIRT1 proteinBackground

SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16). SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.

for Recombinant SIRT1 proteinApplication Notes

Recombinant SIRT1 is suitable for use in histone deacetylase assays. A recommended starting point for the HDAC assay is 25-100 ng of recombinant protein per reaction.

for Recombinant SIRT1 proteinProtein Details

SIRT1 is a histone and protein deacetylase, an NAD-dependant enzyme that removes acetyl groups from lysine acetylated proteins. SIRT1 has been shown to deacetylate histone H4 at lysine 16. Recombinant human SIRT1 was expressed in E. coli as the full length protein (accession number AAD40849) with an N-terminal FLAG-Tag. The molecular weight of the protein is ~120 kDa.

 
SIRT1 protein Coomassie gel

Recombinant SIRT1 protein gel.
SIRT1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

SIRT1 activity assay

Recombinant SIRT1 activity assay
25 ng of acetylated p53 (α-Acp53) was incubated with SIRT1 protein in reaction buffer including 40mM Tris pH 8.0, 5 mM MgCl2, 2mM NAD, and 140 mM KCl for 2 hour at 30c.

for Recombinant SIRT1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SIRT1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SIRT1 protein
 

Data Thumbnails

SIRT1 protein Coomassie gel

SIRT1 protein Coomassie gel.
(Click image to enlarge and see details).

SIRT1 activity assay

SIRT1 activity assay.
(Click image to enlarge and see details).