Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
    

Recombinant PRMT4 (CARM1) protein

Aliases: Mouse Carm1, Mouse Prmt4
商品コード: 31347 Format: 20 µg ¥73,000 Buy Now
商品コード: 31747 Format: 1 mg ¥648,000 Buy Now
Expressed In: Baculovirus Protein Species: Mouse

Request a quote for a bulk orderBulk Order Button


for Recombinant PRMT4 (CARM1) proteinContents

Full length recombinant mousePRMT4 (CARM1) protein supplied in 25 mM HEPES (pH 7.5), 300 mM NaCl, 5% glycerol and 0.2 mg/ml 3X FLAG peptide.

for Recombinant PRMT4 (CARM1) proteinBackground

PRMT4 (CARM1) (Coactivator-associated arginine methyltransferase 1 or Carm1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. PRMT4 (CARM1) methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.

for Recombinant PRMT4 (CARM1) proteinApplication Notes

Recombinant Mouse PRMT4 (CARM1) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 30 ng/μl Histone H3.1 was incubated with 30 ng/μl recombinant PRMT4 (CARM1) protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography.

for Recombinant PRMT4 (CARM1) proteinProtein Details

PRMT4 (CARM1) is protein arginine methyltransferase specific for histone H3. Recombinant mouse PRMT4 (CARM1) was expressed in Sf9 cells (accession number NP_067506) with an N-terminal FLAG-Tag. The molecular weight of the protein is ~67 kDa.

 
Recombinant PRMT4 (CARM1) Coomassie gel

Recombinant PRMT4 (CARM1) protein gel.
PRMT4 (CARM1) protein was run on an SDS-PAGE gel and stained with Coomassie blue.

PRMT4 (CARM1) activity assay

PRMT4 (CARM1) activity assay.
HMT assay measuring the activity of Recombinant PRMT4 (CARM1). Activity was detected by fluorography.

PRMT4 (CARM1) activity assay

PRMT4 (CARM1) activity assay.
Recombinant PRMT4 (CARM1) activity measured using a demethylation assay. MALDI-TOF was used for detection.

for Recombinant PRMT4 (CARM1) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PRMT4 (CARM1) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PRMT4 (CARM1) protein
 

Data Thumbnails

Recombinant PRMT4 (CARM1) Coomassie gel

PRMT4 (CARM1) Coomassie stained gel.
(Click image to enlarge and see details).

PRMT4 (CARM1) activity assay

PRMT4 (CARM1) activity assay.
(Click image to enlarge and see details).

PRMT4 (CARM1) activity assay

PRMT4 (CARM1) activity assay.
(Click image to enlarge and see details).