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Recombinant HDAC3 complex

Aliases: HD3; RPD3; RPD3-2
商品コード: 31349 Format: 50 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC3 complexContents

50 µg recombinant HDAC3/SMRT complex supplied in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC3 complexBackground

The HDAC3/SMRT subunits are members of the NCoR2 co-repressor complex that functions to mediate transcriptional silencing for various transcription factor pathways. HDAC3 is a Class I histone deacetylase with broad specificity. SMRT (also known as NCoR2) is a transcriptional coregulatory protein that binds nuclear receptors and aids in the recruitment of histone deacetylases to DNA promoter regions. Together, the HDAC3/SMRT complex functions to assist nuclear receptors in the downregulation of target gene expression.

for Recombinant HDAC3 complexApplication Notes

The HDAC3/SMRT complex is suitable for use in histone deactylase assays. Specific Activity: 3000 pmol/min/μg. It can also be used to study enzyme kinetics, inhibitor screening, and selectivity profiling. Assay conditions: Prepare 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 20 μM HDAC substrate and HDAC3/SMRT complex. Incubate for 30 minutes at 37°C.

for Recombinant HDAC3 complexProtein Details

The Recombinant HDAC3 complex, active consists of the HDAC3/SMRT subunits of the NcoR2 corepressor complex. Recombinant full length HDAC3 (accession number NM_003883) was co-expressed in a baculovirus expression system with a C-terminal His-Tag (MW = 49.7 kDa) along with amino acids 395-489 of SMRT/NcoR2 (accession number NM_006312) containing an N-terminal GST-Tag (MW = 37.6 kDa).

 
Recombinant HDAC3 complex Coomassie gel

SDS-PAGE gel of Recombinant HDAC3/SMRT protein complex. Lane 1: 1 µg HDAC3/SMRT. Arrows indicate the HDAC3 and SMRT subunits. Lane 2: Protein Marker

Recombinant HDAC3 complex activity assay

HDAC3/SMRT activity assay.
Recombinant HDAC3/SMRT activity measured using a fluorescent histone deacetylase assay.

for Recombinant HDAC3 complexStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC3 complexGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC3 complex
 

Data Thumbnails

Recombinant HDAC3 complex Coomassie gel

Recombinant HDAC3 complex Coomassie stained gel.
(Click image to enlarge and see details)

Recombinant HDAC3 complex activity assay

Recombinant HDAC3 complex activity assay.
(Click image to enlarge and see details).