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Recombinant NCOR2 (SMRT) protein

Aliases: NCOR2; TRAC; CTG26; SMRTE; TRAC1; N-CoR2; TNRC14; TRAC-1; SMAP270; SMRTE-tau
商品コード: 31366 Format: 100 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant NCOR2 (SMRT) proteinContents

100 µg of recombinant NCOR2 (SMRT) protein expressed in E. coli and provided in 50 mM Tris pH 8.0, 150 mM NaCl, 1mM DTT and 50% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant NCOR2 (SMRT) proteinBackground

NCOR2 (SMRT), is a transcriptional corepressor that associates with a subset of nuclear receptors including RAR, PR, GR and TR in the absence of ligand. It is part of a large co-repressor protein complex that includes SIN3A/B and histone deacetylases HDAC1 and HDAC2. The deacetylase activating domain (DAD) functions to bind and activate histone deacetylase 3 (HDAC3) and is also required for N-CoR interaction with HDAC3 and its corepressor activity. SMRT and N-CoR mediated transcriptional corepression plays a significant role in development and metabolism and aberrant corepressor activity is associated with the pathogenesis of myeloid leukemias.

for Recombinant NCOR2 (SMRT) proteinApplication Notes

Recombinant NCOR2 (SMRT) is suitable for use in histone deacetylase (HDAC) assays as it is sufficient for activation of certain HDACs, including HDAC3. Use equimolar with the HDAC used in the assay.

for Recombinant NCOR2 (SMRT) proteinProtein Details

NCOR2 (SMRT) is a transcriptional corepressor that associates with a subset of nuclear receptors including RAR, PR, GR and TR in the absence of ligand. The deacetylase activating domain (DAD) corresponding to amino acids 420 - 480 of NCOR2 (SMRT) (accession number Q9Y618.2) was expressed in E. coli and has an apparent molecular weight of 9 kDa. An epitope tag present during expression was removed proteolytically after purification.

 
NCOR2 (SMRT) Coomassie gel.

NCOR2 (SMRT) protein gel.
NCOR2 (SMRT) run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant NCOR2 (SMRT) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant NCOR2 (SMRT) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant NCOR2 (SMRT) protein
 

Data Thumbnails

NCOR2 (SMRT) Coomassie gel.

NCOR2 (SMRT) Coomassie stained gel.
(Click image to enlarge and see details).