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リコンビナントタンパク質・酵素

Recombinant PHIP (1302-1434) protein

Aliases: ndrp; BRWD2; WDR11; DCAF14
商品コード: 31369 Format: 100 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant PHIP (1302-1434) proteinContents

100 µg of recombinant PHIP (1302-1434) protein expressed in E. coli and supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 0.04% Triton X-100, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PHIP (1302-1434) proteinBackground

PH-interacting protein (PHIP), also known as WDR11 or BRWD2, is a member of the WD repeat protein family. This family is characterized by regions of approximately 40 amino acids bracketed by gly-his and trp-asp (GH-WD), that potentially facilitate the formation of heterotrimeric or multimeric protein complexes. They also contain bromodomains that recognize acetylated histone lysine residues and function as ‘readers’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PHIP was isolated as a factor that binds to the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS-1). PHIP was determined not to be a substrate of the insulin receptor but may instead regulate the insulin and insulin-like growth factor signaling pathways by linking IRS-1 to the insulin receptor. PHIP also stimulates cell proliferation through regulation of cyclin transcription and anti-apoptotic activity through AKT1 phosphorylation and activation. PHIP has also been shown to regulate cell morphology and cytoskeletal organization. In addition, PHIP has been implicated as a positive regulator of beta cell mitogenesis and survival.

for Recombinant PHIP (1302-1434) proteinApplication Notes

Recombinant PHIP (1302-1434) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant PHIP (1302-1434) proteinProtein Details

The peptide corresponding to amino acids 1302 - 1434 that contains the bromodomain sequences of PHIP (accession number NP_060404.3) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 21.994 kDa.

 
Recombinant PHIP (1302-1434) protein gel.

Recombinant PHIP (1302-1434) protein gel.
PHIP (1302-1434) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

HTRF assay for Recombinant PHIP (1302-1434) activity

HTRF assay for Recombinant PHIP (1302-1434) protein activity.
3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

HTRF assay for Recombinant PHIP (1302-1434) activity

HTRF assay for Recombinant PHIP (1302-1434) protein activity.
3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant PHIP (1302-1434) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PHIP (1302-1434) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PHIP (1302-1434) protein
 

Data Thumbnails

Recombinant PHIP (1302-1434) protein gel.

Recombinant PHIP (1302-1434) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF assay for Recombinant PHIP (1302-1434) activity

HTRF assay for Recombinant PHIP (1302-1434) activity.
(Click to enlarge and view details).

HTRF assay for Recombinant PHIP (1302-1434) activity

HTRF assay for Recombinant PHIP (1302-1434) activity.
(Click to enlarge and view details).