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リコンビナントタンパク質・酵素

Recombinant PCAF (715-829) protein

Aliases: K (lysine) acetyltransferase 2B (KAT2B), P300/CBP-associated factor, P/CAF
商品コード: 31370 Format: 100 µg ¥73,000 Buy Now
商品コード: 31770 Format: 1 mg ¥648,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant PCAF (715-829) proteinContents

Recombinant PCAF (715-829) protein expressed in E. coliand supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PCAF (715-829) proteinBackground

P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT’s transactivating activity and may help induce chromatin remodeling of proviral genes.

for Recombinant PCAF (715-829) proteinApplication Notes

Recombinant PCAF (715-829) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant PCAF (715-829) proteinProtein Details

The peptide corresponding to amino acids 715 - 829 that contains the bromodomain sequences of PCAF (accession number NM_003884.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.8 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K36, H4K8, H4K16, H4K20 and TATK50. The recombinant protein is >90% pure by SDS-PAGE.

 
Recombinant PCAF (715-829) protein gel.

Recombinant PCAF (715-829) protein gel.
PCAF (715-829) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant PCAF (715-829) protein activity assay.

HTRF Assay for Recombinant PCAF (715-829) protein activity.
3 µM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with PCAF (715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature, Anti-Flag antibody was used to detect reaction products.

Recombinant PCAF (715-829) protein activity assay.

HTRF Assay for Recombinant PCAF (715-829) protein activity.
3 µM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with PCAF (715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature, Anti-Flag antibody was used to detect reaction products.

for Recombinant PCAF (715-829) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PCAF (715-829) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PCAF (715-829) protein
 

Data Thumbnails

Recombinant PCAF (715-829) protein gel.

Recombinant PCAF (715-829) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

Recombinant PCAF (715-829) protein activity assay.

Recombinant PCAF (715-829) protein activity assay.
(Click image to enlarge and see details).

Recombinant PCAF (715-829) protein activity assay.

Recombinant PCAF (715-829) protein activity assay.
(Click image to enlarge and see details).