Recombinant PCAF (715-829) protein

for Recombinant PCAF (715-829) proteinContents

Recombinant PCAF (715-829) protein expressed in E. coliand supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PCAF (715-829) proteinBackground

P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT’s transactivating activity and may help induce chromatin remodeling of proviral genes.

for Recombinant PCAF (715-829) proteinApplication Notes

Recombinant PCAF (715-829) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant PCAF (715-829) proteinProtein Details

The peptide corresponding to amino acids 715 - 829 that contains the bromodomain sequences of PCAF (accession number NM_003884.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.8 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K36, H4K8, H4K16, H4K20 and TATK50. The recombinant protein is >90% pure by SDS-PAGE.

for Recombinant PCAF (715-829) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PCAF (715-829) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.