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リコンビナントタンパク質・酵素

Recombinant KAT2A (GCN5) (726-837) protein

Aliases: KAT2A; hGCN5; GCN5L2; PCAF-b
商品コード: 31371 Format: 100 µg ¥73,000 Buy Now
商品コード: 31771 Format: 1 mg ¥537,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant KAT2A (GCN5) (726-837) proteinContents

Recombinant KAT2A (GCN5) (726-837) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant KAT2A (GCN5) (726-837) proteinBackground

GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. KAT2A (GCN5) acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests KAT2A (GCN5) may play a role in the induction of chromatin remodeling of proviral genes. KAT2A (GCN5) also functions as a repressor of NFκB by promoting ubiquitination of the NFκB subunit RelA in a HAT-independent manner. KAT2A (GCN5) contains a bromodomain that recognizes acetylated histone lysine residues and functions as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

for Recombinant KAT2A (GCN5) (726-837) proteinApplication Notes

Recombinant KAT2A (GCN5) (726-837) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant KAT2A (GCN5) (726-837) proteinProtein Details

The peptide corresponding to amino acids 726 - 837 that contains the bromodomain sequences of KAT2A (GCN5) (accession number NM_021078.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.4 kDa. It shows binding specificity for acetylated H2AK5, H3K9, H3K14, H3K9/K14, H4K8/K16, H4K16 and H4K5/8/12/16.

 
Recombinant KAT2A (GCN5) (726-837) protein gel.

Recombinant KAT2A (GCN5) (726-837) protein gel.
GCN5 (726-837) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW:19.4 kDa
Purity: > 90%

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) protein activity.

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity.
3 µM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) protein activity.

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity.
3 µM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant KAT2A (GCN5) (726-837) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KAT2A (GCN5) (726-837) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant KAT2A (GCN5) (726-837) protein
 

Data Thumbnails

Recombinant KAT2A (GCN5) (726-837) protein gel.

Recombinant KAT2A (GCN5) (726-837) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) protein activity.

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) protein activity.
(Click image to enlarge and see details).

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) protein activity.

HTRF Assay for Recombinant KAT2A (GCN5) (726-837) protein activity.
(Click image to enlarge and see details).