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リコンビナントタンパク質・酵素

Recombinant CECR2 (425-538) protein

商品コード: 31374 Format: 100 µg ¥73,000 Buy Now
商品コード: 31774 Format: 1 mg ¥481,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant CECR2 (425-538) proteinContents

Recombinant CECR2 (425-538) protein was expressed in E. coli cells and is supplied in 25 mM Tris-HCl pH 8.0, 500 mM NaCl, 0.04% Triton X-100, 20% glycerol, 1mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant CECR2 (425-538) proteinBackground

CECR2 protein is the bromodomain-containing subunit of the CERF (CECR2-containing-remodeling factor) complex. Bromodomains recognize acetylated histone lysine residues and function as ‘readers’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. The CERF complex functions to facilitate the perturbation of chromatin structure in an ATP-dependent manner. In addition to its chromatin remodeling function, CECR2 interaction with LRPPRC may be involved in the integration of the cytoskeletal network with processes such as vesicular trafficking, nucleocytosolic shuttling, transcription, chromosome remodeling and cytokinesis.

for Recombinant CECR2 (425-538) proteinProtein Details

The peptide corresponding to amino acids 425 - 538 that contains the bromodomain sequences of CECR2 (accession number NM_031413.3) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.7 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K9/K14 and H3K9/S10/K14. The recombinant protein is >90% pure by SDS-PAGE.

 
CECR2 (425-538) Coomassie gel

Recombinant CECR2 (425-538) protein gel.
CECR2 (425-538) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

CECR2 (425-538) activity assay

Recombinant CECR2 (425-538) activity assay.
3 µM histone peptide H4K5/8/12/16(ac4) was incubated with CECR2 (425-538) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant CECR2 (425-538) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant CECR2 (425-538) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant CECR2 (425-538) protein
 

Data Thumbnails

CECR2 (425-538) Coomassie gel

CECR2 (425-538) Coomassie stained gel.
(Click image to enlarge and see details).

CECR2 (425-538) activity assay

CECR2 (425-538) activity assay.
(Click image to enlarge and see details).