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リコンビナントタンパク質・酵素

Recombinant PBRM1 (464-605) protein

Aliases: BRG1-associated factor 180 (BAF180), Polybromo 1, PB1; BAF180
商品コード: 31384 Format: 100 µg ¥73,000 Buy Now
商品コード: 31784 Format: 1 mg ¥648,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant PBRM1 (464-605) proteinContents

Recombinant PBRM1 (464-605) protein expressed in E. coli and supplied in 25 mM Tris-HCl pH 8.0, 500 mM NaCl 0.04% Triton X-100, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PBRM1 (464-605) proteinBackground

Polybromo 1 (PBRM1) protein, also known as BRG1-associated factor 180 (BAF180) is the chromatin targeting subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex that is involved in transcriptional activation and repression of select genes. PBRM1 targets chromatin via its six bromodomains that recognize and bind acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. PBRM1 also acts as an inhibitor of cell proliferation and demonstrates tumor suppressing properties in relation to breast, liver and lung cancers.

for Recombinant PBRM1 (464-605) proteinApplication Notes

Recombinant PBRM1 (464-605) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant PBRM1 (464-605) proteinProtein Details

The peptide corresponding to amino acids 464 - 605 that contains the bromodomain sequences of PBRM1 (accession number NM_018165.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 22.5 kDa. It shows binding specificity for acetylated H3K23.

 
PBRM1 (464-605) Coomassie gel

Recombinant PBRM1 (464-605) protein gel.
PBRM1 (464-605) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

PBRM1 (464-605) HTRF activity assay

HTRF Assay for Recombinant PBRM1 (464-605) activity.
3 µM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (464-605) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.

PBRM1 (464-605) HTRF activity assay

HTRF Assay for Recombinant PBRM1 (464-605) activity.
3 µM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (464-605) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.

for Recombinant PBRM1 (464-605) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PBRM1 (464-605) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PBRM1 (464-605) protein
 

Data Thumbnails

PBRM1 (464-605) Coomassie gel

PBRM1 (464-605) Coomassie stained gel.
(Click image to enlarge and see details).

PBRM1 (464-605) HTRF activity assay

PBRM1 (464-605) HTRF activity assay.
(Click image to enlarge and see details).

PBRM1 (464-605) HTRF activity assay

PBRM1 (464-605) HTRF activity assay.
(Click image to enlarge and see details).