Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
リコンビナントタンパク質・酵素

Recombinant DNMT3B / DNMT3L complex

Aliases: DNA Methyltransferase 3 Like, DNA Methyltransferase 3 Beta
商品コード: 31416 Format: 20 µg ¥77,000 Buy Now
商品コード: 31816 Format: 1 mg ¥703,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant DNMT3B / DNMT3L complexContents

Full length recombinant DNMT3B / DNMT3L complex is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant DNMT3B / DNMT3L complexBackground

DNA methyltransferase proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3.

The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. DNMT3L is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation.

for Recombinant DNMT3B / DNMT3L complexApplication Notes

Recombinant DNMT3B / DNMT3L complex is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C.

for Recombinant DNMT3B / DNMT3L complexProtein Details

Recombinant DNMT3B / DNMT3L complex that includes full length human DNMT3B protein (accession number NP_787045.1) with an N-terminal 6×His-Tag and DNMT3L (accession number NP_787063.1) with an N-terminal 6×His-Tag and FLAG-Tag was expressed in Sf9 cells. The molecular weights of DNMT3B and DNMT3L are 89.7 kDa and 48.6 kDa, respectively. The recombinant protein is >90% pure by SDS-PAGE.

 
DNMT3B / DNMT3L complex Coomassie gel

Recombinant DNMT3B / DNMT3L complex gel
Recombinant DNMT3B / DNMT3L complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.

DNMT3B / DNMT3L complex DNMT assay

Recombinant DNMT3B / DNMT3L complex activity assay
Recombinant DNMT3B / DNMT3L complex activity was measured using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C

for Recombinant DNMT3B / DNMT3L complexStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant DNMT3B / DNMT3L complexGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant DNMT3B / DNMT3L complex
 

Data Thumbnails

DNMT3B / DNMT3L complex Coomassie gel

DNMT3B / DNMT3L complex Coomassie gel.
(Click image to enlarge and see details).

DNMT3B / DNMT3L complex DNMT assay

DNMT3B / DNMT3L complex DNMT assay.
(Click image to enlarge and see details).