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リコンビナントタンパク質・酵素

Recombinant KMT2A (MLL1)-SET protein

Aliases: HRX; KMT2A; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL-AF9; MLL/GAS7; TET1-MLL
商品コード: 31419 Format: 50 µg ¥73,000 Buy Now
商品コード: 31819 Format: 1 mg ¥592,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant KMT2A (MLL1)-SET proteinContents

20 µg of Recombinant KMT2A (MLL1)-SET expressed in E. coli and supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant KMT2A (MLL1)-SET proteinBackground

Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), or MLL, also known as KMT2A or HRX, is a Trithorax-group protein that function collectively to promote gene expression. MLL is a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The protein contains multiple conserved functional domains. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.

for Recombinant KMT2A (MLL1)-SET proteinApplication Notes

Recombinant KMT2A (MLL1)-SET is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant KMT2A (MLL1)-SET proteinProtein Details

The peptide corresponding to amino acids 3735 - 3973 of the KMT2A (MLL1) protein (accession number NP_001184033.1) was expressed in E. coli. The peptide contains the SET domain of MLL that is responsible for methyltransferase activity. Recombinant MLL / HRX - SET contains an N-terminal GST-Tag with a molecular weight of 53.7 kDa. The recombinant protein is >92% pure by SDS-PAGE.

 
Recombinant KMT2A (MLL1)-SET Coomassie gel

Recombinant KMT2A (MLL1)-SET protein gel.
MLL / HRX - SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant KMT2A (MLL1)-SET binding assay

Recombinant KMT2A (MLL1)-SET binding assay.
KMT2A (MLL1)-SET was incubated with 4 equimolar components of MLL1 complex (ASH1L, RBBP5, WDR5 and DPY30) for 2 hours at 4°C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant KMT2A (MLL1)-SET proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KMT2A (MLL1)-SET proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant KMT2A (MLL1)-SET protein
 

Data Thumbnails

Recombinant KMT2A (MLL1)-SET Coomassie gel

Recombinant KMT2A (MLL1)-SET Coomassie stained gel.
(Click image to enlarge and see details).

Recombinant KMT2A (MLL1)-SET binding assay

Recombinant KKMT2A (MLL1)-SET.
(Click image to enlarge and see details).