Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
リコンビナントタンパク質・酵素

Recombinant Tet3 (824-1795) protein

Aliases: hCG_40738
商品コード: 31421 Format: 10 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant TET3 (824-1795) proteinContents

Recombinant TET3 (824-1795) protein is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant TET3 (824-1795) proteinBackground

TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation.

TET3 plays an important role in DNA methylation reprogramming processes in the mammalian zygote. TET3 may also contribute to somatic cell nuclear reprogramming during animal cloning. Both of CXXC domain and catalytic domain are important for TET3 regulating key genes of Xenopus eye and neural development.

for Recombinant TET3 (824-1795) proteinApplication Notes

Recombinant TET3 (824-1795) protein is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.

TET Activity Assay Conditions: 250 nM of 30bp DNA oligos (sequence: 5'-CAGTAACTGTGGTC(mC)GGTAACTGACTTGCA-3', contains a 5mCpG site) was incubated with 100 nM recombinant TET3 (824-1795) protein in buffer containing 50 mM HEPES, pH 8.0, 100 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, 1 mM alpha-ketoglutarate, 1 mM ATP, 1 mM TCEP at 37C for 1 hr. Activity was detected by Dot-blot.

for Recombinant TET3 (824-1795) proteinProtein Details

Recombinant TET3 (824-1795) protein that includes amino acids 824-1795 of human TET3 protein (accession number NP_001274420.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 111.3 kDa. The recombinant protein is >30% pure by SDS-PAGE.

 
TET3 Coomassie gel

Recombinant TET3 (824-1795) protein gel.
Recombinant TET3 (824-1795) protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

TET3 activity assay

Recombinant TET3 (824-1795) protein activity assay
Recombinant TET3 (824-1795) protein activity was measured using TET activity assay and detected by Dot-blot.

for Recombinant TET3 (824-1795) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TET3 (824-1795) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Tet3 (824-1795) protein
 

Data Thumbnails

TET3 Coomassie gel

TET3 Coomassie gel.
(Click image to enlarge and see details).

TET3 activity assay

TET3 activity assay.
(Click image to enlarge and see details).