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Recombinant MLL4-SET protein

Aliases: HRX2; MLL2; KMT2B; TRX2; WBP7; MLL1B; WBP-7; CXXC10
商品コード: 31422 Format: 50 µg ¥73,000 Buy Now
商品コード: 31822 Format: 1 mg ¥592,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant MLL4-SET proteinContents

20 µg of recombinant MLL4-SET protein expressed in E. coli and supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant MLL4-SET proteinBackground

Myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4) is a Trithorax-group protein that function collectively to promote gene expression. MLL4 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in β-globin locus transcription regulation by being recruited by Nfe2. MLL4 is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines.

for Recombinant MLL4-SET proteinApplication Notes

Recombinant MLL4-SET is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant MLL4-SET proteinProtein Details

The peptide corresponding to amino acids 2551 - 2715 of the MLL4 protein (accession number NP_055542.1) was expressed in E. coli. The peptide contains the SET domain of MLL2 that is responsible for methyltransferase activity. Recombinant MLL4-SET contains an N-terminal GST-Tag with a molecular weight of 45.2 kDa.

 
MLL4-SET Coomassie gel

Recombinant MLL4-SET protein gel.
MLL4-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

MLL4-SET binding assay

Recombinant MLL4-SET protein gel.
MLL4-SET was incubated 4 equimolar components of the MLL4 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours at 4°C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant MLL4-SET proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant MLL4-SET proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant MLL4-SET protein
 

Data Thumbnails

MLL4-SET Coomassie gel

MLL4-SET Coomassie stained gel.
(Click image to enlarge and see details).

MLL4-SET binding assay

MLL4-SET binding assay.
(Click image to enlarge and see details).