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リコンビナントタンパク質・酵素

Recombinant JMJD1B / KDM3B protein

Aliases: Lysine Demethylase 3B, Jumonji Domain-Containing Protein 1B, Nuclear Protein 5qNCA
商品コード: 31429 Format: 20 μg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant JMJD1B / KDM3B proteinContents

Full length recombinant JMJD1B / KDM3B protein expressed in Sf9 cells and supplied in 25 mM HEPES pH 7.5, 120 mM NaCl, 0.2 mg/ml 3 X FLAG peptide and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant JMJD1B / KDM3B proteinBackground

Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.

for Recombinant JMJD1B / KDM3B proteinApplication Notes

Recombinant JMJD1B / KDM3B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: H3K9me2 demethylase.

Catalytic Ability: > 160 turnovers / enzyme molecule

Histone Demethylase Assay Conditions: 3.3 μM H3K9me2 peptide was incubated with different concentrations of recombinant JMJD1B / KDM3B protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 1 hours at room temperature. HTRF and MALDI-TOF were used for detection.

for Recombinant JMJD1B / KDM3B proteinProtein Details

Recombinant JMJD1B / KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 195.4 kDa. The recombinant protein is >85% pure by SDS-PAGE.

for Recombinant JMJD1B / KDM3B proteinReferences

This protein is described in the following references:
Kim JY et al. (2012) Mol Cell Biol 32:2917-33. doi: 10.1128/MCB.00133-12.

 
JMJD1B / KDM3B Coomassie gel

Recombinant JMJD1B / KDM3B protein gel.
JMJD1B / KDM3B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

JMJD1B / KDM3B activity assay

Recombinant JMJD1B / KDM3B protein activity assay.
3.3 μM H3K9me2 peptide was incubated with 40 nM JMJD1B / KDM3B in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 μM H3K9me2 peptide was used as negative control.

Catalytic Ability: >160 turnovers/ enzyme molecule

JMJD1B / KDM3B activity assay

Recombinant JMJD1B / KDM3B protein activity assay.
3.3 μM H3K9me2 peptide was incubated with recombinant JMJD1B / KDM3B protein in reaction buffer for 1 hours at room temperature. JMJD1B / KDM3B enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody.

for Recombinant JMJD1B / KDM3B proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant JMJD1B / KDM3B proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant JMJD1B / KDM3B protein
 

Data Thumbnails

JMJD1B / KDM3B Coomassie gel

JMJD1B / KDM3B Coomassie stained gel.
(Click image to enlarge and see details).

JMJD1B / KDM3B activity assay

JMJD1B / KDM3B activity assay.
(Click image to enlarge and see details).

JMJD1B / KDM3B activity assay

JMJD1B / KDM3B activity assay.
(Click image to enlarge and see details).