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リコンビナントタンパク質・酵素

Recombinant BAZ1B (1340-1457) protein

Aliases: WSTF; WBSCR9; WBSCR10
商品コード: 31444 Format: 100 μg ¥73,000 Buy Now
商品コード: 31844 Format: 1 mg ¥481,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BAZ1B (1340-1457) proteinContents

Recombinant BAZ1B (1340-1457) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BAZ1B (1340-1457) proteinBackground

Bromodomain adjacent to zinc finger domain, 1B (BAZ1B) is a member of the bromodomain protein family. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BAZ1B is an atypical tyrosine protein kinase that plays a central role in chromatin remodeling and acts as a transcriptional regulator. It is an essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes and has a role in RNA polymerase I and RNA polymerase III transcription. In the complex, BAZ1B mediates the recruitment of the WICH complex to replication foci during DNA replication. BAZ1B and the WICH complex are also involved in DNA damage response through phosphorylation of Tyr142 of histone H2AX (H2AXY142ph), a mark that distinguishes between apoptotic and repair responses to genotoxic stress. BAZ1B is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, BAZ1B plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Deletion of BAZ1B leads to Williams-Beuren syndrome.

for Recombinant BAZ1B (1340-1457) proteinApplication Notes

Recombinant BAZ1B (1340-1457) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BAZ1B (1340-1457) proteinProtein Details

The protein corresponding to amino acids 1340-1457 that contains the bromodomain sequence of human BAZ1B protein (accession number NP_115784.1) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 20.2 kDa. The purity of the protein is > 95% by SDS-PAGE.

 
Recombinant BAZ1B (1340-1457) protein gel.

Recombinant BAZ1B (1340-1457) protein gel.
BAZ1B (1340-1457) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW: 20.2 kDa
Purity: > 95%

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.
3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with BAZ1B (1340-1457) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.
3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with BAZ1B (1340-1457) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant BAZ1B (1340-1457) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BAZ1B (1340-1457) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BAZ1B (1340-1457) protein
 

Data Thumbnails

Recombinant BAZ1B (1340-1457) protein gel.

Recombinant BAZ1B (1340-1457) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.
(Click to enlarge and view details).

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.

HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity.
(Click to enlarge and view details).