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リコンビナントタンパク質・酵素

Recombinant BPTF / FALZ (2791-2911) protein

Aliases: FAC1; NURF301
商品コード: 31447 Format: 100 μg ¥73,000 Buy Now
商品コード: 31847 Format: 1 mg ¥481,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BPTF / FALZ (2791-2911) proteinContents

Recombinant BPTF / FALZ (2791-2911) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BPTF / FALZ (2791-2911) proteinBackground

BPTF (Bromodomain PHD finger transcription factor) is a histone-binding component of NuRF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. BPTF protein contains a DNA-binding domain, a zinc finger motif and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as ‘readers’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. BPTF also specifically recognizes H3 tails trimethylated on lysine 4 (H3K4me3), which mark transcription start sites of virtually all active genes. It may also regulate transcription through direct binding to DNA or transcription factors.

for Recombinant BPTF / FALZ (2791-2911) proteinApplication Notes

Recombinant BPTF / FALZ (2791-2911) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BPTF / FALZ (2791-2911) proteinProtein Details

Recombinant BPTF / FALZ (2791-2911) protein that includes amino acids 2791-2911 of human FALZ protein (accession number NP_872579.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 20.3 kDa. The purity of the protein is >90% by SDS-PAGE.

 
Recombinant BPTF / FALZ (2791-2911) protein gel.

Recombinant BPTF / FALZ (2791-2911) protein gel.
BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW: 20.3 kDa
Purity: > 90%

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.

for Recombinant BPTF / FALZ (2791-2911) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BPTF / FALZ (2791-2911) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BPTF / FALZ (2791-2911) protein
 

Data Thumbnails

Recombinant BPTF / FALZ (2791-2911) protein gel.

Recombinant BPTF / FALZ (2791-2911) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.
(Click image to enlarge and see details).

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.

HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.
(Click image to enlarge and see details).