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Recombinant BRDT (21-137) protein
| Aliases: CT9, BRD6 |
| Catalog No: 31450 | Format: 100 µg | ¥88,000 | Buy |
| Catalog No: 31850 | Format: 1 mg | ¥570,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Application Notes
Recombinant BRDT (21-137) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Protein Details
The peptide corresponding to amino acids 21-137 that contains the bromodomain sequences of BRDT (accession number NP_001717.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 20 kDa. It shows binding specificity for acetylated H2BK12/15, H3K9/14/18/23, H4K12, H4K5/8, H4K8/12, H4K12/16, H4K5/12, H4K5/8/12/16 and H4K5/8/12/16.
Recombinant BRDT (21-137) protein gel.
BRDT (21-137) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
Recombinant BRDT (21-137) HTRF activity assay.
3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (21-137) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
