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Recombinant FBXL10 / KDM2B protein
| Aliases: CXXC2; Fbl10; PCCX2; JHDM1B |
| Catalog No: 31455 | Format: 20 µg | ¥94,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Application Notes
Recombinant FBXL10 / KDM2B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Histone Demethylase Assay Conditions:
50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 300 nM Recombinant FBXL10 / KDM2B protein, and 3.3 μM H3K36me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Protein Details
Recombinant FBXL10 / KDM2B was expressed in a baculovirus expression system as the full length proteins (accession number NP_115979.3) with an N-terminal 6xHis tag and FLAG-Tag. The molecular weight of FBXL10 / KDM2B is 158.2 kDa.
Recombinant FBXL10 / KDM2B protein gel.
FBXL10 / KDM2B protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
FBXL10 / KDM2B activity assay.
Recombinant FBXL10 / KDM2B activity measured by incubating 300 nM FB XL10/KDM2B protein with 3.3 µM H3K36me2 peptide in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 µM H3K36me2 peptide was used as a negative control.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
