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リコンビナントタンパク質・酵素

Recombinant Histone Octamer (H3.3) - biotinylated

商品コード: 31473 Format: 50 µg ¥87,000 Buy Now
商品コード: 31773 Format: 1 mg ¥648,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant Histone Octamer (H3.3) - biotinylatedContents

Recombinant Histone Octamer (H3.3) - biotinylated is supplied in 10 mM Tris-HCl pH 7.4, 2 M NaCl, 1 mM EDTA, 10% glycerol, 5 mM b-mercaptoethanol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Histone Octamer (H3.3) - biotinylatedBackground

The Histone octamer is the eight protein complex found at the center of a nucleosome core particle. It consists of two copies of each of the four core histone proteins (H2A, H2B, H3 and H4). The octamer assembles when a tetramer, containing two copies of both H3 and H4, complexes with two H2A/H2B dimers. Each histone has both an N-terminal tail and a C-terminal histone-fold. Both of these key components interact with DNA in their own way through a series of weak interactions, including hydrogen bonds and salt bridges.

Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.

for Recombinant Histone Octamer (H3.3) - biotinylatedApplication Notes

Recombinant Histone Octamer (H3.3) - biotinylated is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant Histone Octamer (H3.3) - biotinylatedProtein Details

Recombinant Histone Octamer (H3.3) - biotinylated consists of two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.3 that includes amino acids 1-136 (end) (accession number NM_005324) with a C-terminal Biotin tag, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). It was generated in E. coli cells and has an observed molecular weight of 110 kDa.

 
Recombinant Histone Octamer (H3.3) - biotinylated, protein gel.

Recombinant Histone Octamer (H3.3) - biotinylated protein gel.
Recombinant Histone Octamer (H3.3) - biotinylated, was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant Histone Octamer (H3.3) - biotinylatedStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone Octamer (H3.3) - biotinylatedGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Histone Octamer (H3.3) - biotinylated
 

Data Thumbnails

Recombinant Histone Octamer (H3.3) - biotinylated, protein gel.

Recombinant Histone Octamer (H3.3) - biotinylated, protein gel.
(Click image to enlarge and see details).