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リコンビナントタンパク質・酵素

Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag

Aliases: SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
商品コード: 31482 Format: 100 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SMARCA4 / BRG1 (1448-1569), GST-TagContents

Recombinant SMARCA4/BRG1 (1448-1569), GST-Tag is supplied in 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SMARCA4 / BRG1 (1448-1569), GST-TagBackground

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA4 contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA4 is part of the large ATP-dependent chromatin remodeling complex SNF/SWI which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Gene mutation causes Rhabdoid Tumor Predisposition Syndrome Type 2. SMARCA4 functions as a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It also interacts with glucocorticoid receptor (GR), TOPBP1 and progesterone receptor (PR) and is a component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Somatic mutations of SMARCA4 have been detected in some cancer cell lines and loss of SMARCA4 is associated with decreased survival in cancer patients.

for Recombinant SMARCA4 / BRG1 (1448-1569), GST-TagApplication Notes

Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant SMARCA4 / BRG1 (1448-1569), GST-TagProtein Details

The peptide corresponding to amino acids 1448-1569 that contains the bromodomain sequences of SMARCA4 (accession number NP_003063.2) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 41.6 kDa. It shows binding specificity for acetylated H2BK5, H3K14 and H3K9/14. The recombinant protein is >90% pure by SDS-PAGE.

 
SMARCA4 / BRG1 (1448-1569), GST-Tag Coomassie gel

Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag protein gel.
SMARCA4 / BRG1 (1448-1569), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.

SMARCA4 / BRG1 (1448-1569) HTRF activity assay

Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA4 / BRG1 (1448-1569) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

SMARCA4 / BRG1 (1448-1569) HTRF activity assay

Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA4 / BRG1 (1448-1569) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

for Recombinant SMARCA4 / BRG1 (1448-1569), GST-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SMARCA4 / BRG1 (1448-1569), GST-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag
 

Data Thumbnails

SMARCA4 / BRG1 (1448-1569), GST-Tag Coomassie gel

SMARCA4 / BRG1 (1448-1569), GST-Tag Coomassie stained gel.
(Click image to enlarge and see details).

SMARCA4 / BRG1 (1448-1569) HTRF activity assay

SMARCA4 / BRG1 (1448-1569) HTRF activity assay.
(Click image to enlarge and see details).

SMARCA4 / BRG1 (1448-1569) HTRF activity assay

SMARCA4 / BRG1 (1448-1569) HTRF activity assay.
(Click image to enlarge and see details).