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リコンビナントタンパク質・酵素

Recombinant BRDT (257-382), GST-Tag

Aliases: CT9, BRD6
商品コード: 31483 Format: 100 µg ¥73,000 Buy Now
商品コード: 31883 Format: 1 mg ¥537,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRDT (257-382), GST-TagContents

Recombinant BRDT (257-382), GST-Tag protein expressed in E. coli and supplied in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRDT (257-382), GST-TagBackground

BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

for Recombinant BRDT (257-382), GST-TagApplication Notes

Recombinant BRDT (257-382), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRDT (257-382), GST-TagProtein Details

The peptide corresponding to amino acids 257-382 that contains the second bromodomain sequences of BRDT (accession number NP_997072.1) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.8 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
BRDT (257-382), GST-Tag protein gel.

Recombinant BRDT (257-382), GST-Tag protein gel.
BRDT (257-382), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.

BRDT (257-382) HTRF activity assay

Recombinant BRDT (257-382) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

BRDT (257-382) HTRF activity assay

Recombinant BRDT (257-382) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

for Recombinant BRDT (257-382), GST-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRDT (257-382), GST-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant BRDT (257-382), GST-Tag
 

Data Thumbnails

BRDT (257-382), GST-Tag protein gel.

BRDT (257-382), GST-Tag Coomassie stained gel.
(Click image to enlarge and see details).

BRDT (257-382) HTRF activity assay

BRDT (257-382) HTRF activity assay.
(Click image to enlarge and see details).

BRDT (257-382) HTRF activity assay

BRDT (257-382) HTRF activity assay.
(Click image to enlarge and see details).