Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
リコンビナントタンパク質・酵素

Recombinant BRPF1 (1077-1205), GST-Tag

Aliases: BR140, Peregrin
商品コード: 31484 Format: 100 µg ¥73,000 Buy Now
商品コード: 31884 Format: 1 mg ¥481,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant BRPF1 (1077-1205), GST-TagContents

Recombinant BRPF1 (1077-1205), GST-Tag protein expressed in E. coli and supplied in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRPF1 (1077-1205), GST-TagBackground

Bromodomain and PHD finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2.
BRPF1 possesses a PWWP (Pro-Trp-Trp-Pro) domain that functions in the association of PWWP domain-containing proteins with chromatin. PWWP domains have been shown to bind DNA and also specifically recognized methylated lysine residues of histones. In doing so they are suspected to be involved in the mediation of the cross-talk between DNA and histone epigenetic marks. Mutations in PWWP domains have been shown to be associated with various human diseases, including cancer.

for Recombinant BRPF1 (1077-1205), GST-TagApplication Notes

Recombinant BRPF1 (1077-1205), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRPF1 (1077-1205), GST-TagProtein Details

The peptide corresponding to amino acids 1077 - 1205 that contains the PWWP domain sequences of BRPF1 (accession number NP_001003694.1) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.8 kDa. The recombinant protein is >92% pure by SDS-PAGE.

 
BRPF1 (1077-1205), GST-Tag Coomassie gel

Recombinant BRPF1 (1077-1205), GST-Tag protein gel.
BRPF1 (1077-1205), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant BRPF1 (1077-1205), GST-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRPF1 (1077-1205), GST-TagGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRPF1 (1077-1205), GST-Tag
 

Data Thumbnails

BRPF1 (1077-1205), GST-Tag Coomassie gel

BRPF1 (1077-1205), GST-Tag Coomassie stained gel.
(Click image to enlarge and see details).